Tag Archives: PubMed

Response to cautious use of platelet as relevant inducer of liver regeneration following partial hepatectomy in patients with metastatic hepatic carcinoma.

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Response to cautious use of platelet as relevant inducer of liver regeneration following partial hepatectomy in patients with metastatic hepatic carcinoma.
Liver Int. 2017 Dec;37(12):1918-1919
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Response to the role of platelets on regenerating liver: Thoughts beyond parenchymal proliferation.

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Response to the role of platelets on regenerating liver: Thoughts beyond parenchymal proliferation.
Liver Int. 2017 Dec;37(12):1917
Authors: Lisman T
PMID: 29149491 [PubMed – in process]

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Public Health Practice and Policy to Improve Child and Adolescent Health in Europe.

Public Health Practice and Policy to Improve Child and Adolescent Health in Europe.
J Pediatr. 2017 Nov;190:293-294.e2
Authors: Jansen D, Saxena S, Azzopardi-Muscat N
PMID: 29144263 [PubMed – in process]

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Probing the mechanical stability of bridged DNA-H-NS protein complexes by single-molecule AFM pulling.

Probing the mechanical stability of bridged DNA-H-NS protein complexes by single-molecule AFM pulling.
Sci Rep. 2017 Nov 10;7(1):15275
Authors: Liang Y, van der Valk RA, Dame RT, Roos WH, Wuite GJL
Abstract
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Feeling younger and identifying with older adults: Testing two routes to maintaining well-being in the face of age discrimination.

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Feeling younger and identifying with older adults: Testing two routes to maintaining well-being in the face of age discrimination.
PLoS One. 2017;12(11):e0187805
Authors: Armenta BM, Stroebe K, Scheib… Continue reading

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Practical Issues in Implementing Whole-Genome-Sequencing in Routine Diagnostic Microbiology.

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Practical Issues in Implementing Whole-Genome-Sequencing in Routine Diagnostic Microbiology.
Clin Microbiol Infect. 2017 Nov 05;:
Authors: Rossen JWA, Friedrich AW, Moran-Gilad J, ESCMID Study Group f… Continue reading

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Sleep and Alzheimer’s disease: A pivotal role for the suprachiasmatic nucleus.

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Sleep and Alzheimer’s disease: A pivotal role for the suprachiasmatic nucleus.
Sleep Med Rev. 2017 Jul 28;:
Authors: Van Erum J, Van Dam D, De Deyn PP
Abstract
Alzheimer’s disease (AD)… Continue reading

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Nonbullous cutaneous pemphigoid: a systematic review.

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Nonbullous cutaneous pemphigoid: a systematic review.
J Am Acad Dermatol. 2017 Nov 01;:
Authors: Lamberts A, Meijer JM, Jonkman MF
Abstract
BACKGROUND: Cutaneous pemphigoid (bullous pe… Continue reading

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Acquiring new N-glycosylation sites in variable regions of immunoglobulin genes by somatic hypermutation is a common feature of autoimmune diseases.

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Acquiring new N-glycosylation sites in variable regions of immunoglobulin genes by somatic hypermutation is a common feature of autoimmune diseases.
Ann Rheum Dis. 2017 Nov 04;:
Authors: Visser A, Ham… Continue reading

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The structural basis of proton driven zinc transport by ZntB.

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The structural basis of proton driven zinc transport by ZntB.
Nat Commun. 2017 Nov 03;8(1):1313
Authors: Gati C, Stetsenko A, Slotboom DJ, Scheres SHW, Guskov A
Abstract
Zinc is an ess… Continue reading

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Estimating the population-level effectiveness of vaccination program in the Netherlands.

Estimating the population-level effectiveness of vaccination program in the Netherlands.
Epidemiology. 2017 Oct 30;:
Authors: van Wijhe M, McDonald SA, de Melker HE, Postma MJ, Wallinga J
Abstract
BAC… Continue reading

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Characterization of the population structure, drug resistance mechanisms and plasmids of the community-associated Enterobacter cloacae complex in China.

Characterization of the population structure, drug resistance mechanisms and plasmids of the community-associated Enterobacter cloacae complex in China.

J Antimicrob Chemother. 2017 Oct 27;:

Authors: Zhou K, Yu W, Cao X, Shen P, Lu H, Luo Q, Rossen JWA, Xiao Y

Abstract
Objectives: To investigate the population structure, drug resistance mechanisms and plasmids of community-associated Enterobacter cloacae complex (CA-ECC) isolates in China.
Methods: Sixty-two CA-ECC isolates collected from 31 hospitals across China were typed by hsp60 typing and MLST. ESBL and AmpC-overexpression phenotype was determined by double-disc synergy test. Replicon typing and conjugation were performed for plasmid analysis. All ESBL-positive isolates and representative conjugants were subjected to detailed characterization by WGS.
Results: Enterobacter hormaechei and Enterobacter kobei were predominant in our collections. MLST distinguished 46 STs with a polyclonal structure. ST591 was the most prevalent clone detected in northern China. Twenty-two isolates (35.5%) were ESBL positive and half of them were E. kobei. ESBL positivity was related to ESBL production (15/22) and to AmpC overexpression (18/22). Core-genome phylogenetic analysis identified intra- and inter-regional dissemination of ESBL-producing E. kobei clones. ESBL producers were exclusively classified as E. hormaechei and E. kobei, and blaCTX-M-3 was the most prevalent ESBL genotype (10/15) detected in four different environments. In the ESBL-positive population, the ESBL producers encoded more drug resistance genes (8-24 genes) by carrying more plasmids (1-3 plasmids) than the non-ESBL-producing isolates, resulting in an inter-group difference in drug susceptibilities. IncHI-type plasmids were prevalent in the ESBL producers (12/15). All IncHI2-type plasmids (n = 11) carried ESBL genes and shared a similar backbone to p09-036813-1A_261 recovered from Salmonella enterica in Canada.
Conclusions: The species-specific distribution, species-dependent ESBL mechanism and endemic plasmids identified in our study highlight the necessity for tailored surveillance of CA-ECC in the future.

PMID: 29088362 [PubMed – as supplied by publisher]

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Clinical and molecular effects of CHD7 in the heart.

Clinical and molecular effects of CHD7 in the heart.
Am J Med Genet C Semin Med Genet. 2017 Oct 31;:
Authors: Corsten-Janssen N, Scambler PJ
Abstract
Heart defects caused by loss-of-function mutations… Continue reading

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Uneasy to talk about costs of stillbirth?

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Uneasy to talk about costs of stillbirth?

BJOG. 2017 Oct 21;:

Authors: Erwich JJHM

Abstract
In this BJOG stillbirth themed issue, Campbell et al BJOG 2018 XX.XXX report on a population-based cost of illness study. Although reports on costs in healthcare are not unusual, and even quality of life studies are incorporating translations of quality of life into money-estimates, one can feel uneasy when talking about money regarding stillbirth. It seems insensitive towards the people suffering from their loss. How can one put a monetary value on a life not yet lived? However, these authors should be congratulated for building upon earlier work in their effort to tackle this difficult and long neglected topic (Heazell et al Lancet 2016;387:604-616). This article is protected by copyright. All rights reserved.

PMID: 29055084 [PubMed – as supplied by publisher]

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Does obesity along with major depression or anxiety lead to higher use of health care and costs? A 6-year follow-up study.

Does obesity along with major depression or anxiety lead to higher use of health care and costs? A 6-year follow-up study.

Eur J Public Health. 2017 Sep 11;:

Authors: Nigatu YT, Bültmann U, Schoevers RA, Penninx BWJH, Reijneveld SA

Abstract
Background: Evidence lacks on whether obesity along with major depression (MD)/anxiety leads to higher health care use (HCU) and health care-related costs (HCC) compared with either condition alone. The objective of the study was to examine the longitudinal associations of obesity, MD/anxiety, and their combination with HCU and HCC.
Methods: Longitudinal data (2004-2013) among N = 2706 persons at baseline and 2-,4-, and 6-year follow-up were collected on obesity, MD/anxiety and HCU.
Results: The combination of obesity and MD/anxiety was associated with an increased risk of primary and specialty care visits, and of hospitalizations, odds ratios (95%-confidence intervals): 1.83 (1.44; 2.34), 1.31 (1.06; 1.61) and 1.79 (1.40; 2.29) compared to non-obese and non-depressed individuals. The primary and specialty care costs were higher in persons with obesity and MD/anxiety than in persons without these conditions, but the relative excess risk due to interactions between obesity and MD/anxiety regarding HCU and HCC were not statistically significant (i.e. no synergistic effect).
Conclusions: Obesity along with MD/anxiety leads to higher HCU and HCC over time. However, the HCC associated with the joint presence of both conditions are not higher than the sum of the HCC due to each condition independently.

PMID: 29020407 [PubMed – as supplied by publisher]

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Saccharide-Containing Dynamic Proteoids.

Saccharide-Containing Dynamic Proteoids.
Chemistry. 2017 Oct 05;:
Authors: Liu Y, Stuart M, Witte M, Buhler E, Hirsch AKH
Abstract
Dynamic proteoids are dynamic covalent analogues of proteins which ca… Continue reading

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Effect of connective tissue grafting on peri-implant tissue in single immediate implant sites: a RCT.

Effect of connective tissue grafting on peri-implant tissue in single immediate implant sites: a RCT.

J Clin Periodontol. 2017 Sep 23;:

Authors: Zuiderveld EG, Meijer HJA, den Hartog L, Vissink A, Raghoebar GM

Abstract
AIM: To assess the effect of connective tissue grafting on the mid-buccal mucosal level (MBML) of immediately placed and provisionalized single implants in the maxillofacial esthetic zone.
MATERIALS AND METHODS: Sixty patients with a failing tooth were provided with an immediately placed and provisionalized implant. During implant placement, patients randomly received either a connective tissue graft from the maxillary tuberosity (n=30, test group) or no graft (n=30, control group). Follow-up visits were at one (T1 ) and twelve months (T12 ) after final crown placement. The primary outcome measure was any change in MBML compared to the pre-operative situation. In addition, gingival biotype, esthetics (using the Pink Esthetic Score-White Esthetic Score), marginal bone level, soft tissue peri-implant parameters and patient satisfaction were assessed.
RESULTS: The mean MBML change at T12 was -0.5±1.1mm in the control group and 0.1±0.8mm in the test group (p=0.03). No significant differences regarding other outcome variables were observed, neither was gingival biotype associated with a gain or loss in MBML.
CONCLUSIONS: This one-year study shows that connective tissue grafting in single, immediately placed and provisionalized implants leads to less recession of the peri-implant soft tissue at the mid-buccal aspect, irrespective of the gingival biotype (www.trialregister.nl: TC3815). This article is protected by copyright. All rights reserved.

PMID: 28941303 [PubMed – as supplied by publisher]

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MR-guided joint reconstruction of activity and attenuation in brain PET-MR.

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MR-guided joint reconstruction of activity and attenuation in brain PET-MR.

Neuroimage. 2017 Sep 13;162:276-288

Authors: Mehranian A, Zaidi H, Reader AJ

Abstract
With the advent of time-of-flight (TOF) PET scanners, joint maximum-likelihood reconstruction of activity and attenuation (MLAA) maps has recently regained attention for the estimation of PET attenuation maps from emission data. However, the estimated attenuation and activity maps are scaled by unknown scaling factors. We recently demonstrated that in hybrid PET-MR, the scaling issue of this algorithm can be effectively addressed by imposing MR spatial constraints on the estimation of attenuation maps using a penalized MLAA (P-MLAA(+)) algorithm. With the advent of simultaneous PET-MR systems, MRI-guided PET image reconstruction has also gained attention for improving the quantitative accuracy of PET images, usually degraded by noise and partial volume effects. The aim of this study is therefore to increase the benefits of MRI information for improving the quantitative accuracy of PET images by exploiting MRI-based anatomical penalty functions to guide the reconstruction of both activity and attenuation maps during their joint estimation. We employed an anato-functional joint entropy penalty function for the reconstruction of activity and an anatomical quadratic penalty function for the reconstruction of attenuation. The resulting algorithm was referred to as P-MLAA(++) since it exploits both activity and attenuation penalty functions. The performance of the P-MLAA algorithms were compared with MLAA and the widely used activity reconstruction algorithms such as maximum likelihood expectation maximization (MLEM) and penalized MLEM (P-MLEM) both corrected for attenuation using a conventional MRI segmentation-based attenuation correction (MRAC) method. The studied methods were evaluated using simulations and clinical studies taking the PET image reconstructed using reference CT-based attenuation maps as a reference. The simulation results showed that the proposed method can notably improve the visual quality of the PET images by reducing noise while preserving structural boundaries and at the same time improving the quantitative accuracy of the PET images. Our clinical reconstruction results showed that the MLEM-MRAC, P-MLEM-MRAC, MLAA, P-MLAA(+) and P-MLAA(++) algorithms result in, on average, quantification errors of -13.5 ± 3.1%, -13.4 ± 3.1%, -2.0 ± 6.5%, -3.0 ± 3.5% and -4.2 ± 3.6%, respectively, in different regions of the brain. In conclusion, whilst the P-MLAA(+) algorithm showed the best overall quantification performance, the proposed P-MLAA(++) algorithm provided simultaneous partial volume and attenuation corrections with only a minor compromise of PET quantification.

PMID: 28918316 [PubMed – as supplied by publisher]

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Controlled, synchronized actuation of microdroplets by gravity in a superhydrophobic, 3D-printed device.

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Controlled, synchronized actuation of microdroplets by gravity in a superhydrophobic, 3D-printed device.

Anal Chim Acta. 2017 Oct 02;988:50-57

Authors: Oomen PE, Mulder JPSH, Verpoorte E, Oleschuk RD

Abstract
Droplet manipulation over open surfaces allows one to perform assays with a large degree of control and high throughput, making them appealing for applications in drug screening or (bio)analysis. However, the design, manufacturing and operation of these systems comes with high technical requirements. In this study we employ a commercial, low-friction, superhydrophobic coating, Ultra-Ever Dry(®), on a 3D-printed microfluidic device. The device features individual droplet compartments, which allow the manipulation of discrete droplets (10-50 μL) actuated by gravity alone. Simply by angling the device to normal in a 3D-printed holder and rocking in a “to and fro”-fashion, a sequence of droplets can be individually transferred to an electrochemical microelectrode detector and then to waste, while preserving the (chronological) order of samples. Multiple biological fluids (i.e. human saliva, urine and rat blood and serum) were successfully tested for compatibility with the device and actuation mechanism, demonstrating low slip angles and high contact angles. Biological matrix (protein) carryover was probed and effectively mitigated by incorporating aqueous rinse droplets as part of the analysis sequence. As a proof-of-concept, the enzyme-coupled, amperometric detection of glucose was carried out on individual rat serum droplets, enabling total analysis in ≈30 min, including calibration. The device is readily customizable, and the integration of droplet generation techniques and other sensor systems for different analytes of interest or applications can be realized in a plug and play fashion.

PMID: 28916103 [PubMed – in process]

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Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase-cyclohexanone monooxygenase fusions.

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Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase-cyclohexanone monooxygenase fusions.

Appl Microbiol Biotechnol. 2017 Sep 15;:

Authors: Aalbers FS, Fraaije MW

Abstract
The combination of redox enzymes for redox-neutral cascade reactions has received increasing appreciation. An example is the combination of an alcohol dehydrogenase (ADH) with a cyclohexanone monooxygenase (CHMO). The ADH can use NADP(+) to oxidize cyclohexanol to form cyclohexanone and NADPH. Both products are then used by CHMO to produce ε-caprolactone. In this study, these two redox-complementary enzymes were fused, to create a self-sufficient bifunctional enzyme that can convert alcohols to esters or lactones. Three different ADH genes were fused to a gene coding for a thermostable CHMO, in both orientations (ADH-CHMO and CHMO-ADH). All six fusion enzymes could be produced and purified. For two of the three ADHs, we found a clear difference between the two orientations: one that showed the expected ADH activity, and one that showed low to no activity. The ADH activity of each fusion enzyme correlated with its oligomerization state. All fusions retained CHMO activity, and stability was hardly affected. The TbADH-TmCHMO fusion was selected to perform a cascade reaction, producing ε-caprolactone from cyclohexanol. By circumventing substrate and product inhibition, a > 99% conversion of 200 mM cyclohexanol could be achieved in 24 h, with > 13,000 turnovers per fusion enzyme molecule.

PMID: 28916997 [PubMed – as supplied by publisher]

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