Category Archives: Mol Nutr Food Res

Partially hydrolyzed whey proteins prevent clinical symptoms in a cow’s milk allergy mouse model and enhance regulatory T and B cell frequencies.






Partially hydrolyzed whey proteins prevent clinical symptoms in a cow’s milk allergy mouse model and enhance regulatory T and B cell frequencies.
Mol Nutr Food Res. 2017 Jul 05;:
Authors: Kiewiet MBG, van Esch BCAM, … Continue reading

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Arabinoxylan activates Dectin-1 and modulates particulate β-glucan induced Dectin-1 activation.






Arabinoxylan activates Dectin-1 and modulates particulate β-glucan induced Dectin-1 activation.

Mol Nutr Food Res. 2015 Sep 23;

Authors: Sahasrabudhe NM, Schols HA, Faas MM, de Vos P

Abstract
SCOPE: Arabinoxylan is one of the most commonly consumed dietary fiber. Immunomodulation by arabinoxylan is documented but the mechanisms by which these immune-effects are accomplished are unknown.
METHODS AND RESULTS: By applying reporter-cell lines for Toll-like receptors (TLRs) and Dectin-1, we demonstrated that arabinoxylan interacts with Dectin-1 receptors and not with TLRs. Arabinoxylan activates Dectin-1 to a similar magnitude as soluble β-glucans. Soluble β-glucans are known to inhibit the particulate β-glucan induced activation of Dectin-1. As arabinoxylan is also soluble, the inhibiting capacity of arabinoxylan on particulate β-glucan activated Dectin-1 cell-lines was studied. It was found that this inhibition was similar to that of soluble β-glucan and was caused predominantly by inhibition of the Dectin-1A transcript-variant. The Dectin-1 inhibitory function of arabinoxylan was further confirmed in human dendritic cells that demonstrated reduced production of IL-10 and TNF-α. The production of the anti-fungal cytokines IL-4 and IL-23 were increased in dendritic cells stimulated with arabinoxylan and particulate β-glucan. In contrast to soluble β-glucan, arabinoxylan did not enhance production of IL-10, TNF-α, and IL-23.
CONCLUSION: Arabinoxylan activates dectin-1 and supports anti-fungal immune responses in human dendritic cells. The mode of action of arabinoxylan is similar but not identical to that of soluble β-glucans. This article is protected by copyright. All rights reserved.

PMID: 26394716 [PubMed – as supplied by publisher]

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Resistant starches differentially stimulate toll-like receptors and attenuate proinflammatory cytokines in dendritic cells by modulation of intestinal epithelial cells.






Resistant starches differentially stimulate toll-like receptors and attenuate proinflammatory cytokines in dendritic cells by modulation of intestinal epithelial cells.

Mol Nutr Food Res. 2015 May 25;

Authors: Bermudez-Brito M, Rösch C, Schols HA, Faas MM, de Vos P

Abstract
SCOPE: Main objectives of this study were 1) to demonstrate direct signaling of starch on human dendritic cells (DCs), 2) to study whether this is mediated by the pattern recognition receptors (PRRs) such as Toll-like receptors (TLRs) and 3) to study whether intestinal epithelial cells (IECs) are involved in modulating the starch induced immune activation of DCs.
METHODS AND RESULTS: Two different types of Resistant Starch, High-maize® 260 (RS2) and Novelose® 330 (RS3) were characterized for their starch content and particle size. Human DCs and reporter cells for TLRs were incubated with starches and analyzed for NF-kB/AP-1 activation. Complex co-culture systems were applied to study the cross talk. High-maize® 260 predominantly binds to TLR2 while Novelose® 330 binds to TLR2 and TLR5. The strong immune-stimulating effects of High-maize® 260 were attenuated by starch-exposed IECs illustrating the regulatory function of IECs. Despite these attenuating effects, DCs kept producing Th1 cytokines.
CONCLUSION: Resistant starch possesses direct signaling capacity on human DCs in a starch type dependent manner. IECs regulate these responses. High-maize® 260 skews towards a more regulatory phenotype in coculture systems of DCs, IEC, and T-cells. This article is protected by copyright. All rights reserved.

PMID: 26015170 [PubMed – as supplied by publisher]

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The impact of dietary fibers on dendritic cell responses IN VITRO is dependent on the differential effects of the fibers on intestinal epithelial cells.






The impact of dietary fibers on dendritic cell responses IN VITRO is dependent on the differential effects of the fibers on intestinal epithelial cells.

Mol Nutr Food Res. 2015 Jan 24;

Authors: Bermudez-Brito M, Sahasrabudhe NM, Rösch C, Schols HA, Faas MM, de Vos P

Abstract
SCOPE:: In the present study the direct interaction of commonly consumed fibers with epithelial or dendritic cells (DC) was studied.
METHODS & RESULTS:: The fibers were characterized for their sugar composition and chain length profile. When in direct contact, fibers activate DCs only mildly. This was different when DCs and fibers were co-cultured together with supernatants from human epithelial cells (Caco-SM). Caco-SM enhanced the production of IL-12, IL-1Ra, IL-6, IL-8, TNF-α, MCP-1, and MIP-1α but this was strongly attenuated by the dietary fibers. This attenuating effect on proinflammatory cytokines was dependent on the interaction of the fibers with Toll-like receptors as it was reduced by Pepinh-myd88. The interaction of GOS, chicory inulin, wheat arabinoxylan, barley β-glucan with epithelial cells and DCs led to changes in the production of the Th1 cytokines in autologous T-cells, while chicory inulin, and barley β-glucan reduced the Th2 cytokines IL-6. The Treg promoting cytokine IL-10 was induced by GOS whereas chicory inulin decreased the IL-10 production.
CONCLUSIONS:: Our results suggest that dietary fibers can modulate the host immune system not only by the recognized mechanism of effects on microbiota but also by direct interaction with the consumer’s mucosa. This modulation is dietary fiber type dependent. This article is protected by copyright. All rights reserved.

PMID: 25620425 [PubMed – as supplied by publisher]

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Effects of chocolate supplementation on metabolic and cardiovascular parameters in ApoE3L mice fed a high-cholesterol atherogenic diet.






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Effects of chocolate supplementation on metabolic and cardiovascular parameters in ApoE3L mice fed a high-cholesterol atherogenic diet.
Mol Nutr Food Res. 2013 Nov;57(11):2039-48
Authors: Yakala GK, … Continue reading






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