Category Archives: Anal Bioanal Chem

Improving surface and defect center chemistry of fluorescent nanodiamonds for imaging purposes-a review.

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Improving surface and defect center chemistry of fluorescent nanodiamonds for imaging purposes-a review.

Anal Bioanal Chem. 2015 Jul 29;

Authors: Nagl A, Hemelaar SR, Schirhagl R

Abstract
Diamonds are widely used for jewelry owing to their superior optical properties accounting for their fascinating beauty. Beyond the sparkle, diamond is highly investigated in materials science for its remarkable properties. Recently, fluorescent defects in diamond, particularly the negatively charged nitrogen-vacancy (NV(-)) center, have gained much attention: The NV(-) center emits stable, nonbleaching fluorescence, and thus could be utilized in biolabeling, as a light source, or as a Förster resonance energy transfer donor. Even more remarkable are its spin properties: with the fluorescence intensity of the NV(-) center reacting to the presence of small magnetic fields, it can be utilized as a sensor for magnetic fields as small as the field of a single electron spin. However, a reproducible defect and surface and defect chemistry are crucial to all applications. In this article we review methods for using nanodiamonds for different imaging purposes. The article covers (1) dispersion of particles, (2) surface cleaning, (3) particle size selection and reduction, (4) defect properties, and (5) functionalization and attachment to nanostructures, e.g., scanning probe microscopy tips. Graphical Abstract We review how diamond surface and defect chemistry can be optimized for different (bio) applications.

PMID: 26220715 [PubMed – as supplied by publisher]

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Dried blood spot analysis of creatinine with LC-MS/MS in addition to immunosuppressants analysis.

Dried blood spot analysis of creatinine with LC-MS/MS in addition to immunosuppressants analysis.

Anal Bioanal Chem. 2014 Dec 27;

Authors: Koster RA, Greijdanus B, Alffenaar JC, Touw DJ

Abstract
In order to monitor creatinine levels or to adjust the dosage of renally excreted or nephrotoxic drugs, the analysis of creatinine in dried blood spots (DBS) could be a useful addition to DBS analysis. We developed a LC-MS/MS method for the analysis of creatinine in the same DBS extract that was used for the analysis of tacrolimus, sirolimus, everolimus, and cyclosporine A in transplant patients with the use of Whatman FTA DMPK-C cards. The method was validated using three different strategies: a seven-point calibration curve using the intercept of the calibration to correct for the natural presence of creatinine in reference samples, a one-point calibration curve at an extremely high concentration in order to diminish the contribution of the natural presence of creatinine, and the use of creatinine-[(2)H3] with an eight-point calibration curve. The validated range for creatinine was 120 to 480 μmol/L (seven-point calibration curve), 116 to 7000 μmol/L (1-point calibration curve), and 1.00 to 400.0 μmol/L for creatinine-[(2)H3] (eight-point calibration curve). The precision and accuracy results for all three validations showed a maximum CV of 14.0 % and a maximum bias of -5.9 %. Creatinine in DBS was found stable at ambient temperature and 32 °C for 1 week and at -20 °C for 29 weeks. Good correlations were observed between patient DBS samples and routine enzymatic plasma analysis and showed the capability of the DBS method to be used as an alternative for creatinine plasma measurement.

PMID: 25542583 [PubMed – as supplied by publisher]

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Determination of venlafaxine and O-desmethylvenlafaxine in dried blood spots for TDM purposes, using LC-MS/MS.

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Determination of venlafaxine and O-desmethylvenlafaxine in dried blood spots for TDM purposes, using LC-MS/MS.

Anal Bioanal Chem. 2014 Feb 4;

Authors: …

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Simultaneous serum desalting and total protein determination by macroporous reversed-phase chromatography.

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Simultaneous serum desalting and total protein determination by macroporous reversed-phase chromatography.

Anal Bioanal Chem. 2013 Apr;405(10):3195-203

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