Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688.

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Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688.

Appl Environ Microbiol. 2014 Feb;80(3):1082-90

Authors: Dijkman WP, Fraaije MW

Abstract

In the search for useful and renewable chemical building blocks, 5-hydroxymethylfurfural (HMF) has emerged as a very promising candidate, as it can be prepared from sugars. HMF can be oxidized to 2,5-furandicarboxylic acid (FDCA), which is used as a substitute for petroleum-based terephthalate in polymer production. On the basis of a recently identified bacterial degradation pathway for HMF, candidate genes responsible for selective HMF oxidation have been identified. Heterologous expression of a protein from Methylovorus sp. strain MP688 in Escherichia coli and subsequent enzyme characterization showed that the respective gene indeed encodes an efficient HMF oxidase (HMFO). HMFO is a flavin adenine dinucleotide-containing oxidase and belongs to the glucose-methanol-choline-type flavoprotein oxidase family. Intriguingly, the activity of HMFO is not restricted to HMF, as it is active with a wide range of aromatic primary alcohols and aldehydes. The enzyme was shown to be relatively thermostable and active over a broad pH range. This makes HMFO a promising oxidative biocatalyst that can be used for the production of FDCA from HMF, a reaction involving both alcohol and aldehyde oxidations.

PMID: 24271187 [PubMed - indexed for MEDLINE]

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Asymmetry in primary auditory cortex activity in tinnitus patients and controls.

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Asymmetry in primary auditory cortex activity in tinnitus patients and controls.

Neuroscience. 2014 Jan 3;256:117-25

Authors: Geven LI, de…

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Concurrent gemcitabine and 3D radiotherapy in patients with stage III unresectable non-small cell lung cancer

Gerald SMA Kerner, Leon FA van Dullemen, Erwin M Wiegman, Joachim Widder, Edwin Blokzijl, Ellen M Driever, John WG van Putten, Jeroen JW Liesker, Tineke EJ Renkema, Remge M Pieterman, Marc JF Mertens, Thijo JN Hiltermann, Harry JM Groen
Radiation Oncology 2014, 9:190 (29 August 2014)

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Lactic Acid Bacteria: embarking on 30 more years of research

Jan Kok, Eric Johansen, Michiel Kleerebezem, Bas Teusink
Microbial Cell Factories 2014, 13:I1 (29 August 2014)

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Acceptance or challenge?

No description abstract

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Development and preclinical evaluation of radioligands for the PET studies of cerebral adenosine A1 and A2A receptors

No description abstract

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Innovations in studying in vivo cell behavior and pharmacology in complex tissues–microvascular endothelial cells in the spotlight.

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Innovations in studying in vivo cell behavior and pharmacology in complex tissues–microvascular endothelial cells in the spotlight.

Cell Tissue Res. 2013…

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Infants with severe respiratory syncytial virus needed less ventilator time with nasal continuous airways pressure then invasive mechanical ventilation.

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Infants with severe respiratory syncytial virus needed less ventilator time with nasal continuous airways pressure then invasive mechanical ventilation.

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Intra-operator and inter-operator reliability of manual and semiautomated measurement of fetal nuchal translucency: a cross sectional study.

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Intra-operator and inter-operator reliability of manual and semiautomated measurement of fetal nuchal translucency: a cross sectional study.

Prenat Diagn….

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Proteomic studies related to genetic determinants of variability in protein concentrations.

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Proteomic studies related to genetic determinants of variability in protein concentrations.

J Proteome Res. 2014 Jan 3;13(1):5-14

Authors: …

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The effect of visual representation style in problem-solving: a perspective from cognitive processes.

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The effect of visual representation style in problem-solving: a perspective from cognitive processes.

PLoS One. 2013;8(11):e80550

Authors: …

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Successful treatment of a patient with symptomatic long QT syndrome type 3 using ranolazine combined with a beta-blocker.

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Successful treatment of a patient with symptomatic long QT syndrome type 3 using ranolazine combined with a beta-blocker.

Int J Cardiol. 2014 Jan…

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The effect of RAAS blockade on the progression of diabetic nephropathy.

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The effect of RAAS blockade on the progression of diabetic nephropathy.

Nat Rev Nephrol. 2014 Feb;10(2):77-87

Authors: Roscioni SS, Heerspink…

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Necrotiserende Parodontale Aandoeningen : Een Literatuuronderzoek naar de Klinische Kenmerken en Predisponerende Factoren

Background: Necrotizing periodontal disease (NPD) includes necrotizing ulcerative
gingivitis (NUG) and necrotizing ulcerative periodontitis (NUP). These are forms of acute
periodontal infection, characterized by pain or discomfort and infection of the periodontium
and related structures. For the last thirthy years little research has been done into NPD and
there has been little attention for NPD in the Dutch scientific journals for dentists and dental
hygienists. NPD is no longer the order of the day in general dental practice, but it does still
exist in The Netherlands. There are no recent figures on the prevalence in The Netherlands. It
is known that the prevalence of NPD in industrialized countries has been decreasing since
thirty years. Just because NPD is not regularly seen in the Dutch dental practice and because
not diagnosing or misdiagnosing NPD has major consequences for oral health, it is important
that dentists and dental hygienists recognize NPD and are informed of the predisposing
factors for NPD. In this study the clinical characteristics of NPD and the predisposing factors
for NPD are examined
Objective: The purpose of this study is to draw the attention of (future) dentists and dental
hygienists once again to the clinical characteristics of NPD and the predisposing factors for
NPD, and therewith to NPD in general. Moreover it is of great importance to point out to
dentist and dental hygienist the underlying problem with NPD, being either psychological or
physical.
Methods: In order to meet the research request of this study, a literature study has been done.
Several databases and searchterms have been used to find articles, relevant to this study. The
results have been assessed in three stages: based on title, abstract and the full content. The
remaining studies should meet the requirements.
Results: Literature describes various clinical characteristics of NPD: bleeding, pain, necrosis
and ulceration, pseudomembrane formation, foetor ex ore, lymphadenopathy, elevated body
temperature/fever, abnormal sensation in the teeth and bad taste in the mouth. In literature the
following predisposing factors for NPD have been found: bad oral hygiene, smoking, stress,
malnutrition, systemic disease, viral infections and altered immune functions. There are no
primary clinical studies on NUP. NUP has the same clinical characteristics as NUG, but these
are extended with infection of the periodontal ligament and alveolar bone, leading to
attachment loss and bone loss.
Conclusions and Discussion: There is little up to date literature on the clinical characteristics
of NPD and the predisposing factors for NPD. Bleeding, pain and necrosis and ulceration are
the three symptoms in literature generally accepted as the primary clinical feautures of NPD.
Foetor ex ore and pseudomembrane formation are the symptoms that subsequently occur most
consistent with NPD and are therefore considered to be the secondary clinical features of
NPD. Predisposing factors for NPD are poor oral hygiene, smoking, stress, malnutrition,
systemic disease and viral infections. The predisposing factors lead to a weakening of local
and/ or systemic defense mechanisms by which a massive invasion of spirochetes and rodshaped
bacteria in the gingival tissues can occur, resulting in a rapid intense inflammatory

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ColoPulse tablets perform comparably in healthy volunteers and Crohn’s patients and show no influence of food and time of food intake on bioavailability.

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ColoPulse tablets perform comparably in healthy volunteers and Crohn’s patients and show no influence of food and time of food intake on bioavailability.

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The role of pathogen-associated molecular patterns in inflammatory responses against alginate based microcapsules.

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The role of pathogen-associated molecular patterns in inflammatory responses against alginate based microcapsules.

J Control Release. 2013 Dec…

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Management of children with autism spectrum disorder in the dental setting: concerns, behavioural approaches and recommendations.

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Management of children with autism spectrum disorder in the dental setting: concerns, behavioural approaches and recommendations.

Med Oral Patol Oral Cir…

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Localization of liposomal mTHPC formulations within normal epithelium, dysplastic tissue, and carcinoma of oral epithelium in the 4NQO-carcinogenesis rat model.

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Localization of liposomal mTHPC formulations within normal epithelium, dysplastic tissue, and carcinoma of oral epithelium in the 4NQO-carcinogenesis rat model.

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Learn and apply: using multi-sensory storytelling to gather knowledge about preferences and abilities of children with profound intellectual and multiple disabilities–three case studies.

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Learn and apply: using multi-sensory storytelling to gather knowledge about preferences and abilities of children with profound intellectual and multiple disabilities–three…

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Appreciation of CT-negative, lumbar puncture-positive subarachnoid haemorrhage: risk factors for presence of aneurysms and diagnostic yield of imaging.

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Appreciation of CT-negative, lumbar puncture-positive subarachnoid haemorrhage: risk factors for presence of aneurysms and diagnostic yield of imaging.

J…

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