Geographical distribution of publications in the scientific field of surgical oncology.
J Surg Oncol. 2013 Dec;108(8):505-7
Authors: Bakker IS,…
Geographical distribution of publications in the scientific field of surgical oncology.
J Surg Oncol. 2013 Dec;108(8):505-7
Authors: Bakker IS,…
Ineffectiveness of reverse wording of questionnaire items: let’s learn from cows in the rain.
PLoS One. 2013;8(7):e68967
Authors: van Sonderen…
Evaluation of mucosal and systemic immune responses elicited by GPI-0100- adjuvanted influenza vaccine delivered by different immunization strategies.
Cost-effectiveness of the prophylactic HPV vaccine: an application to the Netherlands taking non-cervical cancers and cross-protection into account.
Prediction of upper limb recovery, general disability, and rehabilitation status by activity measurements assessed by accelerometers or the Fugl-Meyer score in acute stroke….
Does loneliness mediate the relation between social support and cognitive functioning in later life?
Soc Sci Med. 2013 Dec;98:116-24
Human herpesvirus-6 DNAemia is a sign of impending primary CMV infection in CMV sero-discordant renal transplantations.
J Clin Virol. 2013…
Shape of the anterior cornea: comparison of height data from 4 corneal topographers.
J Cataract Refract Surg. 2013 Oct;39(10):1570-80
Authors: de Jong T, Sheehan MT, Dubbelman M, Koopmans SA, Jansonius NM
PURPOSE: To compare the ability of clinical corneal topographers to describe the shape of the anterior cornea for optical modeling.
SETTING: University Medical Center Groningen, Groningen, Netherlands.
DESIGN: Cross-sectional study.
METHODS: The anterior corneal shape of healthy subjects was assessed with 4 topographers (Atlas Placido disk, Galilei dual Scheimpflug, Orbscan scanning slit, Pentacam single Scheimpflug). Exported height data were fit with Zernike polynomials. Mean values with the standard deviation, interdevice variability, and test-retest variability were determined for the defocus Z(2,0), astigmatism Z(2,-2) and Z(2,2), coma Z(3,-1) and Z(3,1), and spherical aberration Z(4,0) coefficients for 5.5 mm and 8.0 mm diameters.
RESULTS: At 5.5 mm, the single Scheimpflug topographer showed the smallest coefficient of repeatability; 0.31 μm for Z(2,0); 0.40 and 0.34 μm for Z(2,-2) and Z(2,2), respectively; 0.15 and 0.11 μm for Z(3,-1) and Z(3,1), respectively; and 0.08 μm for Z(4,0); the other topographers showed up to 10 times larger coefficients of repeatability. The (unsigned) mean differences between the topographers were in the range of 0.20 to 1.21 μm for Z(2,0); 0.02 to 0.31 μm and 0.06 to 0.42 μm for Z(2,-2) and Z(2,2), respectively; 0.03 to 0.18 μm and 0.03 to 0.35 μm for Z(3,-1) and Z(3,1), respectively; and 0.00 to 0.14 μm for Z(4,0). The Placido-disk topographer and single Scheimpflug topographer data corresponded best. Similar trends were found at 8.0 mm.
CONCLUSION: Test-retest variability hampered a detailed description of the anterior corneal shape at the level of individual subjects; interdevice variability compromises the exchangeability of the devices.
FINANCIAL DISCLOSURE: No author has a financial or proprietary interest in any material or method mentioned.
PMID: 23945029 [PubMed - indexed for MEDLINE]
A new component of the Nasonia sex determining cascade is maternally silenced and regulates transformer expression.
PLoS One. 2013;8(5):e63618
Authors: Verhulst EC, Lynch JA, Bopp D, Beukeboom LW, van de Zande L
Although sex determination is a universal process in sexually reproducing organisms, sex determination pathways are among the most highly variable genetic systems found in nature. Nevertheless, general principles can be identified among the diversity, like the central role of transformer (tra) in insects. When a functional TRA protein is produced in early embryogenesis, the female sex determining route is activated, while prevention of TRA production leads to male development. In dipterans, male development is achieved by prevention of female-specific splicing of tra mRNA, either mediated by X-chromosome dose or masculinizing factors. In Hymenoptera, which have haplodiploid sex determination, complementary sex determination and maternal imprinting have been identified to regulate timely TRA production. In the parasitoid Nasonia, zygotic transformer (Nvtra) expression and splicing is regulated by a combination of maternal provision of Nvtra mRNA and silencing of Nvtra expression in unfertilized eggs. It is unclear, however, if this silencing is directly on the tra locus or whether it is mediated through maternal silencing of a trans-acting factor. Here we show that in Nasonia, female sex determination is dependent on zygotic activation of Nvtra expression by an as yet unknown factor. This factor, which we propose to term womanizer (wom), is maternally silenced during oogenesis to ensure male development in unfertilized eggs. This finding implicates the upstream recruitment of a novel gene in the Nasonia sex determining cascade and supports the notion that sex determining cascades can rapidly change by adding new components on top of existing regulators.
PMID: 23717455 [PubMed - indexed for MEDLINE]
Impact of chronic respiratory symptoms in a rural area of sub-Saharan Africa: an in-depth qualitative study in the Masindi district of Uganda.
Prim Care Respir J. 2013 Sep;22(3):300-5
Authors: van Gemert F, Chavannes N, Nabadda N, Luzige S, Kirenga B, Eggermont C, de Jong C, van der Molen T
BACKGROUND: Chronic obstructive pulmonary disease (COPD), once regarded as a disease of developed countries, is now recognised as a common disease in low- and middle-income countries. No studies have been performed to examine how the community in resource poor settings of a rural area in sub-Saharan Africa lives with chronic respiratory symptoms.
AIMS: To explore beliefs and attitudes concerning health (particularly respiratory illnesses), use of biomass fuels, tobacco smoking, and the use of health services.
METHODS: A qualitative study was undertaken in a rural area of Masindi district in Uganda, using focus group discussions with 10-15 members of the community in 10 randomly selected villages.
RESULTS: Respiratory symptoms were common among men, women, and children. In several communities respiratory symptoms were stigmatised and often associated with tuberculosis. Almost all the households used firewood for cooking and the majority cooked indoors without any ventilation. The extent of exposure to tobacco and biomass fuel smoke was largely determined by their cultural tradition and gender, tribal origin and socioeconomic factors. Many people were unaware of the damage to respiratory health caused by these risk factors, notably the disproportionate effect of biomass smoke in women and children.
CONCLUSIONS: The knowledge of chronic respiratory diseases, particularly COPD, is poor in the rural community in sub-Saharan Africa. The lack of knowledge has created different beliefs and attitudes concerning respiratory symptoms. Few people are aware of the relation between smoke and respiratory health, leading to extensive exposure to mostly biomass-related smoke.
PMID: 23817677 [PubMed - indexed for MEDLINE]
PML nuclear bodies and SATB1 are associated with HLA class I expression in EBV+ Hodgkin lymphoma.
PLoS One. 2013;8(8):e72930
Authors: Liu Y, van den Berg A, Veenstra R, Rutgers B, Nolte I, van Imhoff G, Visser L, Diepstra A
Tumor cells of classical Hodgkin lymphoma (cHL) are characterized by a general loss of B cell phenotype, whereas antigen presenting properties are commonly retained. HLA class I is expressed in most EBV+ cHL cases, with an even enhanced expression in a proportion of the cases. Promyelocytic leukemia protein (PML) and special AT-rich region binding protein 1 (SATB1) are two global chromatin organizing proteins that have been shown to regulate HLA class I expression in Jurkat cells. We analyzed HLA class I, number of PML nuclear bodies (NBs) and SATB1 expression in tumor cells of 54 EBV+ cHL cases and used 27 EBV- cHL cases as controls. There was a significant difference in presence of HLA class I staining between EBV+ and EBV- cases (p<0.0001). We observed normal HLA class I expression in 35% of the EBV+ and in 19% of the EBV- cases. A stronger than normal HLA class I expression was observed in approximately 40% of EBV+ cHL and not in EBV- cHL cases. 36 EBV+ cHL cases contained less than 10 PML-NBs per tumor cell, whereas 16 cases contained more than 10 PML-NBs. The number of PML-NBs was positively correlated to the level of HLA class I expression (p<0.01). The percentage of SATB1 positive cells varied between 0% to 100% in tumor cells and was inversely correlated with the level of HLA class I expression, but only between normal and strong expression (p<0.05). Multivariable analysis indicated that the number of PML-NBs and the percentage of SATB1+ tumor cells are independent factors affecting HLA class I expression in EBV+ cHL. In conclusion, both PML and SATB1 are correlated to HLA class I expression levels in EBV+ cHL.
PMID: 24009715 [PubMed - indexed for MEDLINE]
Critical factors in the translation of improved antimicrobial strategies for medical implants and devices.
Biomaterials. 2013 Dec;34(37):9237-43
Authors: Grainger DW, van der Mei HC, Jutte PC, van den Dungen JJ, Schultz MJ, van der Laan BF, Zaat SA, Busscher HJ
Biomaterials-associated infection incidence represents an increasing clinical challenge as more people gain access to medical device technologies worldwide and microbial resistance to current approaches mounts. Few reported antimicrobial approaches to implanted biomaterials ever get commercialized for physician use and patient benefit. This is not for lack of ideas since many thousands of claims to new approaches to antimicrobial efficacy are reported. Lack of translation of reported ideas into medical products approved for use, results from conflicting goals and purposes between the various participants involved in conception, validation, development, commercialization, safety and regulatory oversight, insurance reimbursement, and legal aspects of medical device innovation. The scientific causes, problems and impressive costs of the limiting clinical options for combating biomaterials-associated infection are well recognized. Demands for improved antimicrobial technologies constantly appear. Yet, the actual human, ethical and social costs and consequences of their occurrence are less articulated. Here, we describe several clinical cases of biomaterials-associated infections to illustrate the often-missing human elements of these infections. We identify the current societal forces at play in translating antimicrobial research concepts into clinical implant use and their often-orthogonal constituencies, missions and policies. We assert that in the current complex environment between researchers, funding agencies, physicians, patients, providers, producers, payers, regulatory agencies and litigators, opportunities for translatable successes are minimized under the various risks assumed in the translation process. This argues for an alternative approach to more effectively introduce new biomaterials and device technologies that can address the clinical issues by providing patients and medical practitioners new options for desperate clinical conditions ineffectively addressed by biomedical innovation.
PMID: 24034505 [PubMed - indexed for MEDLINE]
[Discharge from hospital: left in limbo].
Ned Tijdschr Geneeskd. 2013;157(32):A6394
Authors: Berendsen AJ
Enhancing majority members’ pro-diversity beliefs in small teams: the facilitating effect of self-anchoring.
Exp Psychol. 2014 Jan 1;61(1):3-11
Inclusion of frail elderly patients in clinical trials: solutions to the problems.
J Geriatr Oncol. 2013 Jan;4(1):26-31
Authors: Hempenius L,…
Fanny Schierenbeck, Maarten W N Nijsten, Anders Franco-Cereceda, Jan Liska
Critical Care 2014, 18:R56 (31 March 2014)
Kim L Brunekreeft, Corinna Strohm, Marloes J Gooden, Anna A Rybczynska, Hans W Nijman, Götz U Grigoleit, Wijnand Helfrich, Edwin Bremer, Daniela Siegmund, Harald Wajant, Marco de Bruyn
Molecular Cancer 2014, 13:85 (17 April 2014)
Metformin protects rat hepatocytes against bile acid-induced apoptosis.
PLoS One. 2013;8(8):e71773
Authors: Woudenberg-Vrenken TE, Conde de la Rosa L, Buist-Homan M, Faber KN, Moshage H
BACKGROUND: Metformin is used in the treatment of Diabetes Mellitus type II and improves liver function in patients with non-alcoholic fatty liver disease (NAFLD). Metformin activates AMP-activated protein kinase (AMPK), the cellular energy sensor that is sensitive to changes in the AMP/ATP-ratio. AMPK is an inhibitor of mammalian target of rapamycin (mTOR). Both AMPK and mTOR are able to modulate cell death.
AIM: To evaluate the effects of metformin on hepatocyte cell death.
METHODS: Apoptotic cell death was induced in primary rat hepatocytes using either the bile acid glycochenodeoxycholic acid (GCDCA) or TNFα in combination with actinomycin D (actD). AMPK, mTOR and phosphoinositide-3 kinase (PI3K)/Akt were inhibited using pharmacological inhibitors. Apoptosis and necrosis were quantified by caspase activation, acridine orange staining and Sytox green staining respectively.
RESULTS: Metformin dose-dependently reduces GCDCA-induced apoptosis, even when added 2 hours after GCDCA, without increasing necrotic cell death. Metformin does not protect against TNFα/ActD-induced apoptosis. The protective effect of metformin is dependent on an intact PI3-kinase/Akt pathway, but does not require AMPK/mTOR-signaling. Metformin does not inhibit NF-κB activation.
CONCLUSION: Metformin protects against bile acid-induced apoptosis and could be considered in the treatment of chronic liver diseases accompanied by inflammation.
PMID: 23951244 [PubMed - indexed for MEDLINE]
Upregulation of endogenous ICAM-1 reduces ovarian cancer cell growth in the absence of immune cells.
Int J Cancer. 2014 Jan 15;134(2):280-90
Authors: de Groote ML, Kazemier HG, Huisman C, van der Gun BT, Faas MM, Rots MG
Ovarian cancer is a difficult-to-treat cancer with a 5-year survival rate of only ∼45%, due to late diagnosis and therapy resistance. In need of new therapeutic approaches, induction of intercellular adhesion molecule (ICAM)-1 expression might be of interest, since the expression of ICAM-1 is lower in ovarian cancer cells compared with healthy ovarian cells and correlated with decreased tumorigenicity. Whereas ICAM-1 expression on tumor cells is of importance for attracting immune cells, ICAM-1 might also induce tumorigenicity and chemoresistance. In ovarian cancer, such a role of ICAM-1 is unclear. Here, we investigated whether ICAM-1 has a cell-biological role by bidirectional modulation of ICAM-1 expression using ICAM-targeting artificial transcription factors. For a panel of ovarian cancer cells, tumor growth and cisplatin sensitivity were evaluated. Induction of ICAM-1 expression (ranging from 3- to 228-fold on mRNA level and 1.7- to 108-fold on protein level) resulted in indications of decreased ovarian cancer cell growth and reduced cisplatin sensitivity. Repression ranged from 48 to 94% on mRNA level and 47 to 91% on protein level. This study shows that, next to its established immunogenic role, ICAM-1 affects cell biological behavior of ovarian cancer cells and, importantly, that reexpression by artificial transcription factors represents a powerful approach for functional validation of genes epigenetically silenced in cancer, such as ICAM-1.
PMID: 23832872 [PubMed - indexed for MEDLINE]