A design for external quality assessment for the analysis of thiopurine drugs: pitfalls and opportunities.

A design for external quality assessment for the analysis of thiopurine drugs: pitfalls and opportunities.

Clin Chem Lab Med. 2018 Aug 15;:

Authors: Robijns K, van Luin M, Jansen RTP, Neef C, Touw DJ

PMID: 30110249 [PubMed – as supplied by publisher]

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Concordance assessment of self-reported medication use in the Netherlands three-generation Lifelines Cohort Study with the pharmacy database IADB.nl: The PharmLines Initiative. Clinical Epidemiology 2018:2010;1-9

Original language English
Pages (from-to) 981-989
Journal Journal of Clinical Epidemiology
Volume 10
State Published – 16-Aug-2018
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The efficacy of computerized cognitive drill and practice training for patients with a schizophrenia-spectrum disorder: A meta-analysis.

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The efficacy of computerized cognitive drill and practice training for patients with a schizophrenia-spectrum disorder: A meta-analysis.

Schizophr Res. 2018 Aug 07;:

Authors: Prikken M, Konings MJ, Lei WU, Begemann MJH, Sommer IEC

Abstract
BACKGROUND: Computerized methods for improving cognitive functioning in schizophrenia have gained popularity during the past decades. Therefore, this study evaluates the available evidence for the efficacy of computerized cognitive drill and practice training for patients with schizophrenia-spectrum disorders.
METHODS: A systematic search was carried out using PubMed, Embase, Cochrane Database of Systematic Reviews, and PsycINFO. A meta-analysis was performed to compare cognitive drill and practice training in patients with a schizophrenia-spectrum disorder with non-cognitively oriented control conditions. The primary outcome was cognitive functioning. Secondary outcome measures included psychotic symptoms, depressive symptoms, and functional outcomes. Effect sizes (ES) for all included studies were calculated as Hedges’ g.
RESULTS: 24 studies were included with 1262 patients in total. Compared to a control condition, patients receiving computerized cognitive drill and practice training showed significantly more improvement on attention (ES = 0.31, p = 0.001), working memory (ES = 0.38, p < 0.001), positive symptoms (ES = 0.31, p = 0.003), and depressive symptoms (ES = 0.37, p = 0.002). Small, marginally significant effect sizes were found for processing speed, verbal and visual learning and memory, and verbal fluency. However, significant effects on functional outcomes and social cognition were absent.
DISCUSSION: The current study showed evidence for the efficacy of computerized cognitive drill and practice training in patients with schizophrenia-spectrum disorders. However, the absence of effects on social cognition and functional outcomes questions the generalization of treatment effects. Together, these results stimulate further development of computerized training programs for schizophrenia that not only improve cognitive functioning, but also generalize cognitive improvement to functional outcomes.

PMID: 30097278 [PubMed – as supplied by publisher]

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A novel in vitro assay reveals SNARE topology and the role of Ykt6 in autophagosome fusion with vacuoles.

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A novel in vitro assay reveals SNARE topology and the role of Ykt6 in autophagosome fusion with vacuoles.

J Cell Biol. 2018 Aug 10;:

Authors: Gao J, Reggiori F, Ungermann C

Abstract
Autophagy is a catabolic pathway that delivers intracellular material to the mammalian lysosomes or the yeast and plant vacuoles. The final step in this process is the fusion of autophagosomes with vacuoles, which requires SNARE proteins, the homotypic vacuole fusion and protein sorting tethering complex, the RAB7-like Ypt7 GTPase, and its guanine nucleotide exchange factor, Mon1-Ccz1. Where these different components are located and function during fusion, however, remains to be fully understood. Here, we present a novel in vitro assay to monitor fusion of intact and functional autophagosomes with vacuoles. This process requires ATP, physiological temperature, and the entire fusion machinery to tether and fuse autophagosomes with vacuoles. Importantly, we uncover Ykt6 as the autophagosomal SNARE. Our assay and findings thus provide the tools to dissect autophagosome completion and fusion in a test tube.

PMID: 30097515 [PubMed – as supplied by publisher]

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Clinical benefit of controversial first line systemic therapies for advanced stage ovarian cancer – ESMO-MCBS scores.

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Clinical benefit of controversial first line systemic therapies for advanced stage ovarian cancer – ESMO-MCBS scores.

Cancer Treat Rev. 2018 Jun 18;69:233-242

Authors: Broekman KE, Jalving M, van Tinteren H, Sessa C, Reyners AKL

Abstract
BACKGROUND: The magnitude of clinical benefit scale (MCBS) was introduced by the European Society of Medical Oncology (ESMO) to quantify the clinical benefit of therapeutic regimens and to prioritise therapies. It distinguishes curative from palliative treatments and ranks their benefit based on overall survival (OS), progression free survival (PFS), quality of life (QoL) and toxicity. Objective of this study on the first line treatment of ovarian cancer was to evaluate the evidence for the current standard of care using the ESMO-MCBSv1.1 with an emphasis on controversial therapeutic options: intraperitoneal chemotherapy, dose-dense paclitaxel and bevacizumab.
METHODS: Phase III trials, published since 1992, investigating first line systemic treatment of Fédération Internationale de Gynécologie et d’Obstétrique (FIGO) stage IIB-IV epithelial ovarian cancer were included. Since most studies included patients with FIGO stage IV disease or incomplete debulking, all treatments were judged to be palliative. Treatments were graded 5 to 1 on the ESMO-MCBSv1.1, where grades 5 and 4 represent a high level of clinical benefit.
RESULTS: 55 studies met the inclusion criteria. ESMO-MCBS scores were calculated for eleven studies that showed a statistically significant benefit of the experimental treatment. Intraperitoneal (ip) cisplatin scored a 4 and 3, but two other studies were negative and therefore not scored on the ESMO-MCBS. Dose-dense paclitaxel showed substantial clinical benefit in one study (score 4), but three studies were negative. Addition of bevacizumab also scored a 4 in one study subgroup including high-risk patients but a 2 in another trial with a larger study population.
CONCLUSION: Based on ESMO-MCBS scores, dose-dense paclitaxel and intraperitoneal chemotherapy cannot be recommended as standard treatment. Bevacizumab should be considered only in the high-risk population. The ESMO-MCBSv1.1. helps to summarise reported studies on controversial treatment regimens, and identifies their weaknesses.

PMID: 30098485 [PubMed – as supplied by publisher]

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Boosting heterologous protein production yield by adjusting global nitrogen and carbon metabolic regulatory networks in Bacillus subtilis.

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Boosting heterologous protein production yield by adjusting global nitrogen and carbon metabolic regulatory networks in Bacillus subtilis.

Metab Eng. 2018 Aug 07;:

Authors: Cao H, Villatoro-Hernandez J, Weme RDO, Frenzel E, Kuipers OP

Abstract
Bacillus subtilis is extensively applied as a microorganism for the high-level production of heterologous proteins. Traditional strategies for increasing the productivity of this microbial cell factory generally focused on the targeted modification of rate-limiting components or steps. However, the longstanding problems of limited productivity of the expression host, metabolic burden and non-optimal nutrient intake, have not yet been completely solved to achieve significant production-strain improvements. To tackle this problem, we systematically rewired the regulatory networks of the global nitrogen and carbon metabolism by random mutagenesis of the pleiotropic transcriptional regulators CodY and CcpA, to allow for optimal nutrient intake, translating into significantly higher heterologous protein production yields. Using a β-galactosidase expression and screening system and consecutive rounds of mutagenesis, we identified mutant variants of both CodY and CcpA that in conjunction increased production levels up to 290%. RNA-Seq and electrophoretic mobility shift assay (EMSA) showed that amino acid substitutions within the DNA-binding domains altered the overall binding specificity and regulatory activity of the two transcription factors. Consequently, fine-tuning of the central metabolic pathways allowed for enhanced protein production levels. The improved cell factory capacity was further demonstrated by the successfully increased overexpression of GFP, xylanase and a peptidase in the double mutant strain.

PMID: 30096425 [PubMed – as supplied by publisher]

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Psychological distress in patients with obstructive sleep apnoea: The role of hostility and coping self-efficacy

Original language English
Number of pages 16
Journal International journal of health psychology
State E-pub ahead of print – 11-Aug-2018
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Vitamin A and beta (β)-carotene supplementation for cystic fibrosis.

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Vitamin A and beta (β)-carotene supplementation for cystic fibrosis.

Cochrane Database Syst Rev. 2018 Aug 09;8:CD006751

Authors: de Vries JJ, Chang AB, Bonifant CM, Shevill E, Marchant JM

Abstract
BACKGROUND: People with cystic fibrosis (CF) and pancreatic insufficiency are at risk of a deficiency in fat-soluble vitamins, including vitamin A. Vitamin A deficiency predominantly causes eye and skin problems, while excessive levels of vitamin A can harm the respiratory and skeletal systems in children and interfere with the metabolism of other fat-soluble vitamins. Most CF centres administer vitamin A as supplements to reduce the frequency of vitamin A deficiency in people with CF and to improve clinical outcomes such as growth, although the recommended dose varies between different guidelines. Thus, a systematic review on vitamin A and vitamin A-like supplementation (carotenes or other retinoids) in people with CF would help guide clinical practice. This is an update of an earlier Cochrane Review.
OBJECTIVES: To determine if supplementation with vitamin A, carotenes or other retinoid supplements in children and adults with CF reduces the frequency of vitamin A deficiency disorders, improves general and respiratory health and affects the frequency of vitamin A toxicity.
SEARCH METHODS: We searched the Cochrane Cystic Fibrosis and Genetic Disorders Group Cystic Fibrosis Trials Register compiled from electronic database searches and handsearching of journals and conference abstract books. Additionally we searched several ongoing trials registries, including ClinicalTrials.gov, the WHO International Clinical Trials Registry Platform and the International Standard Randomised Controlled Trial Number Registry.Most recent database searches: 01 June 2018.
SELECTION CRITERIA: All randomised or quasi-randomised controlled studies comparing all preparations of oral vitamin A, carotenes or retinoids (or in combination), used as a supplement compared to placebo at any dose, for at least three months, in people with CF (diagnosed by sweat tests or genetic testing) with and without pancreatic insufficiency.
DATA COLLECTION AND ANALYSIS: Two authors individually assessed study quality and extracted data on outcome measures. The authors assessed the quality of the evidence using the GRADE system. Investigators were contacted to retrieve missing quantitative data.
MAIN RESULTS: No studies of vitamin A or other retinoid supplementation were eligible for inclusion. However, one randomised study of beta (β)-carotene supplementation involving 24 people with CF who were receiving pancreatic enzyme substitution was included. The study compared successive β-carotene supplementation periods (high dose followed by low dose) compared to placebo. The results for the low-dose supplementation period should be interpreted with caution, due to the lack of a wash-out period after the high-dose supplementation.The included study did not report on two of the review’s primary outcomes (vitamin A deficiency disorders and mortality); results for our third primary outcome of growth and nutritional status (reported as z score for height) showed no difference between supplementation and placebo, mean difference (MD) -0.23 (95% confidence interval (CI) -0.89 to 0.43) (low-quality evidence). With regards to secondary outcomes, supplementation with high-dose β-carotene for three months led to significantly fewer days of systemic antibiotics required to treat pulmonary exacerbations, compared to controls, MD -15 days (95% CI -27.60 to -2.40); however, this was not maintained in the second three-month section of the study when the level of β-carotene supplementation was reduced, MD -8 days (95% CI -18.80 to 2.80) (low-quality evidence). There were no statistically significant effects between groups in lung function (low-quality evidence) and no adverse events were observed (low-quality evidence). Supplementation affected levels of β-carotene in plasma, but not vitamin A levels. The study did not report on quality of life or toxicity.
AUTHORS’ CONCLUSIONS: Since no randomised or quasi-randomised controlled studies on retinoid supplementation were identified, no conclusion on the supplementation of vitamin A in people with CF can be drawn. Additionally, due to methodological limitations in the included study, also reflected in the low-quality evidence judged following the specific evidence grading system (GRADE), no clear conclusions on β-carotene supplementation can be drawn. Until further data are available, country- or region-specific guidelines regarding these practices should be followed.

PMID: 30091146 [PubMed – as supplied by publisher]

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Reaction to “Intergrating smoking cessation care in alcohol and other drug treatment settings using an organisational change intervention: a systematic review” by Skelton, et al.

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Reaction to “Intergrating smoking cessation care in alcohol and other drug treatment settings using an organisational change intervention: a systematic review” by Skelton, et al.

Addiction. 2018 Aug 09;:

Authors: Van de Graaf RC, Veldman K, Anthonio GG

PMID: 30091225 [PubMed – as supplied by publisher]

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Clinical outcome and risk factors for failure in late acute prosthetic joint infections treated with debridement and implant retention.

Clinical outcome and risk factors for failure in late acute prosthetic joint infections treated with debridement and implant retention.

J Infect. 2018 Aug 06;:

Authors: Wouthuyzen-Bakker M, Sebillotte M, Lomas J, Taylor A, Palomares EB, Murillo O, Parvizi J, Shohat N, Reinoso JC, Sánchez RE, Fernandez-Sampedro M, Senneville E, Huotari K, Allende JMB, Garcia-Cañete J, Lora-Tamayo J, Ferrari MC, Vaznaisiene D, Yusuf E, Aboltins C, Trebse R, Salles MJ, Benito N, Vila A, Toro MDD, Kramer TS, Petersdorf S, Diaz-Brito V, Tufan ZK, Sanchez M, Arvieux C, Soriano A, ESCMID Study Group for Implant-Associated Infections (ESGIAI)

Abstract
OBJECTIVES: Debridement, antibiotics and implant retention (DAIR) is the recommended treatment for all acute prosthetic joint infections (PJI), but its efficacy in patients with late acute (LA) PJI is not well described.
METHODS: Patients diagnosed with LA PJI between 2005 and 2015 were retrospectively evaluated. LA PJI was defined as the development of acute symptoms (≤ 3 weeks) occurring ≥ 3 months after arthroplasty. Failure was defined as: i) the need for implant removal, ii) infection related death, iii) the need for suppressive antibiotic therapy and/or iv) relapse or reinfection during follow-up.
RESULTS: 340 patients from 27 centers were included. The overall failure rate was 45.0% (153/340). Failure was dominated by Staphylococcus aureus PJI (54.7%, 76/139). Significant independent preoperative risk factors for failure according to the multivariate analysis were: fracture as indication for the prosthesis (odds ratio (OR) 5.4), rheumatoid arthritis (OR 5.1), age above 80 years (OR 2.6), male gender (OR 2.0) and C-reactive protein >150 mg/L (OR 2.0). Exchanging the mobile components during DAIR was the strongest predictor for treatment success (OR 0.35).
CONCLUSION: LA PJIs have a high failure rate. Treatment strategies should be individualized according to patients’ age, comorbidity, clinical presentation and microorganism causing the infection.

PMID: 30092305 [PubMed – as supplied by publisher]

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A novel in vitro assay reveals SNARE topology and the role of Ykt6 in autophagosome fusion with vacuoles

A novel in vitro assay reveals SNARE topology and the role of Ykt6 in autophagosome fusion with vacuoles

Gao, J., Reggiori, F. & Ungermann, C. 10-Aug-2018 In : The Journal of Cell Biology. 13 p.

Research output: Contribution to journalArticle

Original language English
Number of pages 13
Journal The Journal of Cell Biology
State E-pub ahead of print – 10-Aug-2018
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A report on the status of vaccination in Europe

A report on the status of vaccination in Europe

Sheikh, S., Biundo, E., Courcier, S., Damm, O., Launay, O., Maes, E., Marcos, C., Matthews, S., Meijer, C., Poscia, A., Postma, M., Saka, O., Szucs, T. & Begg, N. 9-Aug-2018 In : Vaccine. 36, 33, p. 4979-4992 14 p.

Research output: Contribution to journalReview article

Original language English
Pages (from-to) 4979-4992
Number of pages 14
Journal Vaccine
Volume 36
Issue number 33
State Published – 9-Aug-2018
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Renal Functional Reserve Capacity before and after Living Kidney Donation

Renal Functional Reserve Capacity before and after Living Kidney Donation

van Londen, M., Kasper, N., Hessels, N. R., Messchendorp, A. L., Bakker, S. J. L., Sanders, J-S. F., Berger, S. P., de Borst, M. H. & Navis, G. 8-Aug-2018 In : American journal of physiology-Renal physiology. 20 p.

Research output: Contribution to journalArticle

Original language English
Number of pages 20
Journal American journal of physiology-Renal physiology
State E-pub ahead of print – 8-Aug-2018
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Single-chain polymer nanoparticles in controlled drug delivery and targeted imaging.

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Single-chain polymer nanoparticles in controlled drug delivery and targeted imaging.

J Control Release. 2018 Aug 02;:

Authors: Kröger APP, Paulusse JMJ

Abstract
As a relatively new class of materials, single-chain polymer nanoparticles (SCNPs) just entered the field of (biomedical) applications, with recent advances in polymer science enabling the formation of bio-inspired nanosized architectures. Exclusive intramolecular collapse of individual polymer chains results in individual nanoparticles. With sizes an order of magnitude smaller than conventional polymer nanoparticles, SCNPs are in the size regime of many proteins and viruses (1-20 nm). Multifaceted syntheses and design strategies give access to a wide set of highly modular SCNP materials. This review describes how SCNPs have been rendered water-soluble and highlights ongoing research efforts towards biocompatible SCNPs with tunable properties for controlled drug delivery, targeted imaging and protein mimicry.

PMID: 30077737 [PubMed – as supplied by publisher]

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Substance use disorders and sexual behavior; the effects of alcohol and drugs on patients’ sexual thoughts, feelings and behavior.

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Substance use disorders and sexual behavior; the effects of alcohol and drugs on patients’ sexual thoughts, feelings and behavior.

Addict Behav. 2018 Jul 06;87:231-237

Authors: Bosma-Bleeker MH, Blaauw E

Abstract
INTRODUCTION: Hardly any research exists on the relationship between substance use and sexual behaviors in patients with a substance use disorder. This study aimed to examine this relation by looking into perceived positive effects on sexual behavior, perceived negative effects and risky sexual behavior due to substance use in patient groups of users of alcohol, stimulants, sedatives and Gamma hydroxybutyrate (GHB). In addition, the current study aimed to address the question whether sexual behavior (e.g. number of sexual partners, sexual activity) differs between these patient groups.
METHOD: A total of 180 patients with a substance use disorder (i.e. alcohol, amphetamine, cannabis, cocaine, GHB and opiates) participated. A self-report questionnaire was administered with questions on substance use, sexual behaviors (e.g. sexual activity, masturbation, use of pornography) and statements about the perceived changes in sexual functioning and behavior under influence of the primary substance of abuse.
RESULTS: All four groups reported changes in sexual thoughts, feelings and behavior due to the use of their primary substance. More than half of the patients reported enhancements in sexual domains (i.e. sexual pleasure, sexual arousal, sexual behavior), but also decrements or risky behaviors and about a quarter stated that their sexual thoughts, feelings and behaviors were often associated with the use of their primary substance of abuse. Patients with a GHB use disorder reported the strongest relation between drug use and sexual behavior. Users of GHB not only reported more enhancement in several sexual domains, but also less decline in sexual domains compared to the other patient groups and more risky behavior or more sexual activity than some of the other groups of patients.
CONCLUSIONS: The results underline the importance of addressing the relationship between substance use and sexual behavior in treatment programs, as patients may be hesitant to stop their use of substances when they experience many positive effects in their sexual behavior. Future research directions are suggested.

PMID: 30077915 [PubMed – as supplied by publisher]

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Imaging before cardiac resynchronisation therapy implantation-luxury or necessity?

Imaging before cardiac resynchronisation therapy implantation-luxury or necessity?

Maass, A. H. & Yap, S. C. 7-Aug-2018 In : Netherlands Heart Hournal. 3 p.

Research output: Contribution to journalArticle

Original language English
Number of pages 3
Journal Netherlands Heart Hournal
State E-pub ahead of print – 7-Aug-2018
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Development of a core outcome set for immunomodulation in pregnancy (COSIMPREG): A protocol for a systematic review and Delphi study

Development of a core outcome set for immunomodulation in pregnancy (COSIMPREG): A protocol for a systematic review and Delphi study

Prins, J. R., Holvast, F., van ‘t Hooft, J., Bos, A. F., Ganzevoort, J. W., Scherjon, S. A., Robertson, S. A. & Gordijn, S. J. 5-Aug-2018 In : BMJ Open. 8, 8, 6 p., e021619

Research output: Contribution to journalArticle

Original language English
Article number e021619
Number of pages 6
Journal BMJ Open
Volume 8
Issue number 8
State Published – 5-Aug-2018
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Determinants of successful lifestyle change during a 6-month preconception lifestyle intervention in women with obesity and infertility

Original language English
Number of pages 13
Journal European journal of nutrition
State E-pub ahead of print – 3-Aug-2018
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Fibronectin aggregates promote features of a classically and alternatively activated phenotype in macrophages

Fibronectin aggregates promote features of a classically and alternatively activated phenotype in macrophages

Sikkema, A. H., Stoffels, J. M. J., Wang, P., Basedow, F. J., Bulsink, R., Bajramovic, J. J. & Baron, W. 2-Aug-2018 In : Journal of neuroinflammation. 15, 218, 17 p.

Research output: Contribution to journalArticle

Original language English
Number of pages 17
Journal Journal of neuroinflammation
Volume 15
Issue number 218
State Published – 2-Aug-2018
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Response to the letter of Liccardi et al

Response to the letter of Liccardi et al

Maters, G. A., Pool, G., Sanderman, R., Wempe, J. B. & Fleer, J. 2-Aug-2018 In : COPD: Journal of Chronic Obstructive Pulmonary Disease. 2 p.

Research output: Contribution to journalComment/Letter to the editor

Original language English
Number of pages 2
Journal COPD: Journal of Chronic Obstructive Pulmonary Disease
State E-pub ahead of print – 2-Aug-2018
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