Response to cautious use of platelet as relevant inducer of liver regeneration following partial hepatectomy in patients with metastatic hepatic carcinoma.
Liver Int. 2017 Dec;37(12):1918-1919
Authors: Lisman T
PMID: 29149488 [PubMed – in process]
Posted in Liver Int
Tagged PubMed, TOP25
Response to the role of platelets on regenerating liver: Thoughts beyond parenchymal proliferation.
Liver Int. 2017 Dec;37(12):1917
Authors: Lisman T
PMID: 29149491 [PubMed – in process]
Posted in Liver Int
Tagged PubMed, TOP25
Public Health Practice and Policy to Improve Child and Adolescent Health in Europe.
J Pediatr. 2017 Nov;190:293-294.e2
Authors: Jansen D, Saxena S, Azzopardi-Muscat N
PMID: 29144263 [PubMed – in process]
Posted in J Pediatr
Tagged PubMed, TOP25
Probing the mechanical stability of bridged DNA-H-NS protein complexes by single-molecule AFM pulling.
Sci Rep. 2017 Nov 10;7(1):15275
Authors: Liang Y, van der Valk RA, Dame RT, Roos WH, Wuite GJL
Atomic force microscopy (AFM) has proven to be a powerful tool for the study of DNA-protein interactions due to its ability to image single molecules at the nanoscale. However, the use of AFM in force spectroscopy to study DNA-protein interactions has been limited. Here we developed a high throughput, AFM based, pulling assay to measure the strength and kinetics of protein bridging of DNA molecules. As a model system, we investigated the interactions between DNA and the Histone-like Nucleoid-Structuring protein (H-NS). We confirmed that H-NS both changes DNA rigidity and forms bridges between DNA molecules. This straightforward methodology provides a high-throughput approach with single-molecule resolution which is widely applicable to study cross-substrate interactions such as DNA-bridging proteins.
PMID: 29127381 [PubMed – in process]
Posted in Sci Rep
Tagged PubMed, TOP25
Feeling younger and identifying with older adults: Testing two routes to maintaining well-being in the face of age discrimination.
PLoS One. 2017;12(11):e0187805
Authors: Armenta BM, Stroebe K, Scheibe S, Postmes T, Van Yperen NW
Integrating the social identity and aging literatures, this work tested the hypothesis that there are two independent, but simultaneous, responses by which adults transitioning into old age can buffer themselves against age discrimination: an individual response, which entails adopting a younger subjective age when facing discrimination, and a collective response, which involves increasing identification with the group of older adults. In three experimental studies with a total number of 488 older adults (50 to 75 years of age), we manipulated age discrimination in a job application scenario and measured the effects of both responses on perceived health and self-esteem. Statistical analyses include individual study results as well as a meta-analysis on the combined results of the three studies. Findings show consistent evidence only for the individual response, which was in turn associated with well-being. Furthermore, challenging previous research, the two responses (adopting a younger subjective age and increasing group identification) were not only theoretically, but also empirically distinct. This research complements prior research by signaling the value of considering both responses to discrimination as complementary rather than mutually exclusive.
PMID: 29117257 [PubMed – in process]
Posted in PLoS One
Tagged PubMed, TOP25
Practical Issues in Implementing Whole-Genome-Sequencing in Routine Diagnostic Microbiology.
Clin Microbiol Infect. 2017 Nov 05;:
Authors: Rossen JWA, Friedrich AW, Moran-Gilad J, ESCMID Study Group for Genomic and Molecular Diagnostics (ESGMD)
BACKGROUND: next generation sequencing (NGS) is increasingly being used in clinical microbiology. Like every new technology that is being adopted in microbiology, the integration of NGS into clinical and routine workflows needs to be carefully managed.
AIM: to review the practical aspects of implementing bacterial whole genome sequencing (WGS) in routine diagnostic laboratories.
SOURCES: review of the literature and expert opinion.
CONTENT: in this review, we discuss when and how to integrate whole genome sequencing (WGS) in the routine workflow of the clinical laboratory. In addition, as the microbiology laboratories have to adhere to various national and international regulations and criteria for their accreditation, we deliberate on quality control issues for using WGS in microbiology, including the importance of proficiency testing. Furthermore, the current and future place of this technology in the diagnostic hierarchy of microbiology is described as well as the necessity of maintaining backwards compatibility with already established methods. Finally, we speculate on the question whether WGS can entirely replace routine microbiology in the future and the tension between the fact that most sequencers are designed to process multiple samples in parallel whereas for optimal diagnosis a one-by-one processing of the samples is preferred. Special reference is made to the cost and turnaround time of WGS in diagnostic laboratories.
IMPLICATIONS: further development is required to improve the workflow for WGS, particularly shorten the turnaround time, reduce costs and streamline downstream data analyses. Only when these processes will reach maturity, reliance on WGS for routine patient management and infection control management will become feasible, enabling the transformation of clinical microbiology into a genome-based and personalised diagnostic field.
PMID: 29117578 [PubMed – as supplied by publisher]
Sleep and Alzheimer’s disease: A pivotal role for the suprachiasmatic nucleus.
Sleep Med Rev. 2017 Jul 28;:
Authors: Van Erum J, Van Dam D, De Deyn PP
Alzheimer’s disease (AD), which accounts for most of the dementia cases, is, aside from cognitive deterioration, often characterized by the presence of non-cognitive symptoms. Society is desperately in need for interventions that alleviate the economic and social burden related to AD. Circadian dysrhythmia, one of these symptoms in particular, immensely decreases the self-care ability of AD patients and is one of the main reasons of caregiver exhaustion. Studies suggest that these circadian disturbances form the root of sleep-wake problems, diagnosed in more than half of AD patients. Sleep abnormalities have generally been considered merely a consequence of AD pathology. Recent evidence suggests that a bidirectional relationship exists between sleep and AD, and that poor sleep might negatively impact amyloid burden, as well as cognition. The suprachiasmatic nucleus (SCN), the main circadian pacemaker, is subjected to several alterations during the course of the disease. Its functional deterioration might fulfill a crucial role in the relation between AD pathophysiology and the development of sleep abnormalities. This review aims to give a concise overview of the anatomy and physiology of the SCN, address how AD pathology precisely impacts the SCN and to what degree these alterations can contribute to the progression of the disease.
PMID: 29102282 [PubMed – as supplied by publisher]
Nonbullous cutaneous pemphigoid: a systematic review.
J Am Acad Dermatol. 2017 Nov 01;:
Authors: Lamberts A, Meijer JM, Jonkman MF
BACKGROUND: Cutaneous pemphigoid (bullous pemphigoid) is an autoimmune bullous disease that typically presents with tense bullae and severe pruritus. However, bullae may be lacking, a subtype termed nonbullous cutaneous pemphigoid.
OBJECTIVE: To summarize the reported characteristics of nonbullous cutaneous pemphigoid.
METHODS: The EMBASE and MEDLINE databases were searched using ‘nonbullous cutaneous pemphigoid’ and various synonyms. Case reports and series describing nonbullous cutaneous pemphigoid were included.
RESULTS: The search identified 133 articles. After selection 39 articles were included, presenting 132 cases. Erythematous, urticarial plaques (52.3%) and papules/nodules (20.5%) were the most reported clinical features. The mean age at presentation was 74.9 years. Histopathology was commonly nonspecific. Linear depositions of IgG/C3 along the basement membrane zone were found by direct immunofluorescence microscopy in 93.2%. Indirect immunofluorescence on salt split skin was positive in 90.2%. The mean diagnostic delay was 22.6 months. The minority of patients (9.8%) developed bullae during the reported follow-up.
LIMITATIONS: Results are mainly based on case reports/small case series.
CONCLUSION: Nonbullous cutaneous pemphigoid is an underdiagnosed variant of pemphigoid that most often does not evolve to bullous lesions, and mimics other pruritic skin diseases. Greater awareness among physicians is needed to avoid delay in diagnosis.
PMID: 29102490 [PubMed – as supplied by publisher]
Acquiring new N-glycosylation sites in variable regions of immunoglobulin genes by somatic hypermutation is a common feature of autoimmune diseases.
Ann Rheum Dis. 2017 Nov 04;:
Authors: Visser A, Hamza N, Kroese FGM, Bos NA
PMID: 29102958 [PubMed – as supplied by publisher]
The structural basis of proton driven zinc transport by ZntB.
Nat Commun. 2017 Nov 03;8(1):1313
Authors: Gati C, Stetsenko A, Slotboom DJ, Scheres SHW, Guskov A
Zinc is an essential microelement to sustain all forms of life. However, excess of zinc is toxic, therefore dedicated import, export and storage proteins for tight regulation of the zinc concentration have evolved. In Enterobacteriaceae, several membrane transporters are involved in zinc homeostasis and linked to virulence. ZntB has been proposed to play a role in the export of zinc, but the transport mechanism of ZntB is poorly understood and based only on experimental characterization of its distant homologue CorA magnesium channel. Here, we report the cryo-electron microscopy structure of full-length ZntB from Escherichia coli together with the results of isothermal titration calorimetry, and radio-ligand uptake and fluorescent transport assays on ZntB reconstituted into liposomes. Our results show that ZntB mediates Zn(2+) uptake, stimulated by a pH gradient across the membrane, using a transport mechanism that does not resemble the one proposed for homologous CorA channels.
PMID: 29101379 [PubMed – in process]
Estimating the population-level effectiveness of vaccination program in the Netherlands.
Epidemiology. 2017 Oct 30;:
Authors: van Wijhe M, McDonald SA, de Melker HE, Postma MJ, Wallinga J
BACKGROUND: There are few estimates of the effectiveness of long-standing vaccination programs in developed countries. To fill this gap, we investigate the direct and indirect effectiveness of childhood vaccination programs on mortality at the population level in the Netherlands.
METHODS: We focused on three communicable infectious diseases, diphtheria, pertussis, and poliomyelitis, for which we expect both direct and indirect effects. As a negative control, we used tetanus, a non-communicable infectious disease for which only direct effects are anticipated. Mortality data from 1903-2012 were obtained from Statistics Netherlands. Vaccination coverage data were obtained from various official reports. For the birth cohorts 1903 through 1975, all-cause and cause-specific childhood mortality burden was estimated using restricted mean lifetime survival methods, and a model was used to describe the pre-vaccination decline in burden. By projecting model results into the vaccination era, we obtained the expected burden without vaccination. Program effectiveness was estimated as the difference between observed and expected mortality burden.
RESULTS: Each vaccination program showed a high overall effectiveness, increasing to nearly 100% within ten birth cohorts. For diphtheria, 14.9% (95% uncertainty interval [UI]: 12.3%, 17.6%) of mortality burden averted by vaccination was due to indirect protection. For pertussis, this was 32.1% (95% UI: 31.3%, 32.8%). No indirect effects were observed for poliomyelitis or tetanus with -2.4% (UI: -16.7%, 7.1%) and 0.6% (UI: -17.9%, 10.7%) respectively.
CONCLUSION: Vaccination programs for diphtheria and pertussis showed substantial indirect effects, providing evidence for herd protection.
PMID: 29087989 [PubMed – as supplied by publisher]